tannin-binding proteins in human saliva
نویسندگان
چکیده
Tannins have a number ofdetrimental biological effects and these include interference with normal growth and metabolism if they are present in the feed of various animals. Proline-rich proteins (PRPs) in saliva have been shown to provide protection against tannin, but little is known about the mechanism of protection and interaction of other salivary proteins with tannin. To identify tannin-binding human salivary proteins, parotid and submandibular/sublingual saliva samples were adsorbed with tannin. PRPs, and in particular a group of low-Mr proteins, were readily precipitated by tannin. The low-Mr proteins were purified from parotid saliva and demonstrated to be histatins, a family of wellcharacterized histidine-rich salivary proteins. The ability of synthetic histatin 5, as well as an acidic PRP (PRP-1) and gelatin to precipitate quebracho condensed tannin and tannic acid was
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